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has been cited by the following article:

• TITLE: Leptin Receptor in Ram Epididymal Spermatozoa

  AUTHORS: Mohammad Roostaei-Ali Mehr, Reza Rajabi-Toustani, Rasool Motamedi-Mojdehi

  KEYWORDS: Epididymal Spermatozoa; mRNA; Ob-R; Ob-Rb

  JOURNAL NAME: Engineering, Vol.4 No.10B, October 21, 2013

  ABSTRACT: This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then they were purified. Specific bands (98 and 308 bp) for Ob-R and Ob-Rb were detected after RT-PCR in both epididymal spermatozoa and placental cotyledon. We may conclude that Ob-R and Ob-Rb mRNA are present in ram epididymal spermatozoa and leptin perhaps exerts physiological effects, as already demonstrated in human.