TITLE:
CD133 and CD24 expression in renal tissue of patients affected by autosomal dominant polcystic kidney disease
AUTHORS:
Daniele Lodi, Giulia Ligabue, Fabrizio Cavazzini, Valentina Lupo, Gianni Cappelli, Riccardo Magistroni
KEYWORDS:
Renal Stem Cells; CD133; CD24; ADPKD
JOURNAL NAME:
Stem Cell Discovery,
Vol.3 No.4,
October
8,
2013
ABSTRACT:
Background: Autosomal
dominant polycystic kidney disease is a condition mainly characterized by the
progressive development and enlargement of cysts in each kid ney. In this
process a high rate of proliferation and apoptosis of tubular cells has been
documented and interpreted as a futile attempt of tissue repair. In
consideration of the role of stem cells in reparative processes we investigated
the presence and localization of CD133 + CD24+ renal progenitors in renal ADPKD
tissue and cells. Methods: Two normal kidneys and two ADPKD kidneys were
examined. CD133 and CD24 expression was investigated by confocal microscopy and
immunoblotting. Furthermore cystic isolated cells and cultured immortalized
cells were characterized. Results: CD133 and CD24 have the same localization in
ADPKD tissues and in normal kidneys: expression is restricted to a subset of
epithelial cells (PEC) of Bowman’s capsule and to tubular cells in a focal and
segmental pattern. Furthermore, in ADPKD tissue, cysts diffusely express CD133
and CD24. According to a quantitative analysis in ADPKD tissue CD133 + CD24 +
cells are statistically more expressed in tubules (p calizations in control tissue. Conclusions: CD133 and CD24 antigens, typically expressed by renal epithelial progenitors, are
more expressed in ADPKD tubules and highly expressed in ADPKD cysts. Whether
CD133 and CD24 expression would signify renal progenitor recruitment or
alternatively an expression pattern of the dedifferentiation of ADPKD cells
remains unclear.