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• TITLE: Reference gene selection for quantitative PCR studies in bovine neutrophils

  AUTHORS: William R. Vorachek, Gerd Bobe, Jean A. Hall

  KEYWORDS: Blood Neutrophils; Bos taurus; qPCR; Reference Genes; Selenium Treatment

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.4 No.10B, September 12, 2013

  ABSTRACT: Reference genes are essential for studying mRNA expression with quantitative PCR (qPCR). We investigated 11 candidate whole-blood neutrophil reference genes (ACTB, B2M, G6PD, GAPDH, GYPC, HPRT, PGK1, RPL19, SDHA, TFRC, and YWHAZ) for beef calves, both males and females, with or without selenium supplementation. Initial screening was based on gene expression level (scoring the need to validate neutrophil reference genes under different experimental conditions. Multiple reference genes rather than a single gene may provide more robust and reliable results. The best pair of reference genes in whole-blood neutrophils from beef calves overall was PGK1|YWHAZ.