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has been cited by the following article:

• TITLE: Rolling Circle Amplification on Biotin-Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces

  AUTHORS: Herin Oh, Alec Cerchiari, Desirée Sørensen, Timothy Mon, Cassandra L. Smith

  KEYWORDS: Cyclic Polyolefin; Ligation Mediated Rolling Circle Amplification; SNP Detection

  JOURNAL NAME: Materials Sciences and Applications, Vol.4 No.9, September 6, 2013

  ABSTRACT: Cyclic polyolefin (COP) is an inexpensive hydrophobic material with low auto-fluorescence, high light transmittance and thermal stability, broad chemical resistance and no non-specific protein binding. Here, the hydrophobic alkane COP was modified to have carbonyl functionalities through oxygen plasma and chemical etching treatments to increase usefulness for chemical and biochemical applications. Then, biotin-hydrazide was used to create biotinylated surfaces that bound streptavidin. A biotinylated target oligonucleotide was subsequently bound to the immobilized biotin-streptavidin and ligation mediated rolling circle amplification-based (L-RCA) SNP detection was demonstrated.