TITLE:
The timing and extent of intraosseous hypoxia in the oxidative stress-induced rat osteonecrosis model
AUTHORS:
Toru Ichiseki, Ayumi Kaneuji, Seiji Kaneko, Syusuke Ueda, Yoshimichi Ueda, Hideto Yonekura, Kiyokazu Fukui, Tadami Matsumoto
KEYWORDS:
Osteonecrosis; Oxidative Stress; Buthionine Sulfoximine; Hypoxia-Inducible Factor-1α
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.8,
August
1,
2013
ABSTRACT:
Using a rat oxidative stress-induced femoral head
osteonecrosis model, we determined the presence/ absence and timing of the
generation of hypoxia in the femoral head. DL-Buthionine-(S,R)-sulfoximine (BSO)
500 mg/kg was administered intraperitoneally to male Wistar rats. The rats were
killed at 1, 3, 6, 12 hours, and 1, 3, 5 days after BSO administration, and the
bilateral femora were removed. A group not administered BSO (control group)
was also studied (each group n = 5). In the femoral heads of each group, the expression
of hypoxia-inducible factor-1 alpha (HIF-1α)
as an index of hypoxia was confirmed by the Western blot method, and quantified
using analytical software. In the femoral head increased HIF-1α expression was found in all groups
from 1 hour after BSO administration (p α amounting to
about 13-fold of that of control group was noted (p