TITLE:
Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type
AUTHORS:
Yah Gwladys Gnamien, Irié Arsène Zoro Bi, Yatty Justin Kouadio, Yves Brostaux, Jean-Pierre Baudoin
KEYWORDS:
AFLP; Genetic Stability; In Vitro; Oleaginous Seeds; Organogenesis
JOURNAL NAME:
Agricultural Sciences,
Vol.4 No.7A,
July
18,
2013
ABSTRACT:
To regenerate
adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations
of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were
tested. Shoot induction proved to depend on the interaction between levels of
sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2
strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with
6 g/l agar allowed the production of numerous shoots without a callus phase.
After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots
with an average of 12.26 shoots per explant and a mean shoot length of 17.13
mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization
were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal
parts ranged from 54% to 96%. Rooted plantlets were acclimatized and
transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of
the in-vitro-regenerated plantlets by
means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets
and mother plants.