Djè, Y., Kouonon, L.C., Zoro Bi, I.A., Gnamien, Y.G. and Baudoin, J.P. (2006) étude des caractéristiques botaniques, agronomiques et de la biologie florale du melon africain (Cucumis melo L. var. agrestis Naudin, Cucurbitaceae) (Study of the botanical, agronomic characteristics and the floral biology of the African melon (Cucumis melo L. var. agrestis Naudin, Cucurbitaceae). Biotechnolgy, Agronomy, Society and Environment, 10, 109-119.

has been cited by the following article:

• TITLE: Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type

  AUTHORS: Yah Gwladys Gnamien, Irié Arsène Zoro Bi, Yatty Justin Kouadio, Yves Brostaux, Jean-Pierre Baudoin

  KEYWORDS: AFLP; Genetic Stability; In Vitro; Oleaginous Seeds; Organogenesis

  JOURNAL NAME: Agricultural Sciences, Vol.4 No.7A, July 18, 2013

  ABSTRACT: To regenerate adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were tested. Shoot induction proved to depend on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar allowed the production of numerous shoots without a callus phase. After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots with an average of 12.26 shoots per explant and a mean shoot length of 17.13 mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal parts ranged from 54% to 96%. Rooted plantlets were acclimatized and transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of the in-vitro-regenerated plantlets by means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets and mother plants.