TITLE:
Mass production, fermentation, formulation of Pseudomonas putida for controlling of die back and phomopsis diseases on grapevine
AUTHORS:
Wafaa M. Haggag, Malaka A. E. Saleh, Inas Mostafa, Noran Adel
KEYWORDS:
Die Back; Phomopsis Diseases; Grapevine and Pseudomonas putida
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.6,
June
24,
2013
ABSTRACT:
The export oriented agricultural and horticultural
crops depends on the export of residue free produce and has created a great
potential and demand for the incorporation of biopesticides in crop protection.
PGPR with wide scope for commercialization includes Pseudomonas putida. Pseudomonas
putida was effective in reducing die back caused by a fungus Botryodiplodia theobromae and phomopsis or
arm death, caused by a fungus, Phomopsis viticola of grapevine in vitro and in vivo. Pseudomonas putida showed optimum Siderophore pseudobactin production
at 72 h, and growth peak at 120 h. glycerol as a source of carbon and yeast as
a source of nitrogen Pseudomonas putida was formulated using polysaccharide dextran starch, which led to the elongation
of conservation and the effectiveness of the biofungicide. Pseudomonas
putida was very effective as biocontrol agents to reduce the die back and phomopsis diseases on grapevine. The
obtained results showed that both bacterial
treatments significantly increased the growth parameters as well as
fruits dry weights and yield of grapevine.