TITLE:
Expression changes of Akt and GSK-3β during vascular inflammatory response and oxidative stress induced by high-fat diet in rats
AUTHORS:
Jingshang Wang, Huijun Yin, Ye Huang, Chunyu Guo, Chengdong Xia, Lu Zhang
KEYWORDS:
High-Fat Diet; Akt and GSK-3β; Vascular Inflammatory Response; Oxidative Stress; Endothelium Injury
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.6 No.5A,
May
21,
2013
ABSTRACT:
Aim: To observe the expression changes of Akt and
GSK-3β during vascular inflammatory
response and oxidative stress induced by high-fat
diet in rats. Methods: 20 male Sprague-Dawley rats were separately fed for 18 weeks with two types of diets; a normal diet
(control group, CON) or high-fat diet hyperlipidmia group, HLP). Then the body
weight, lipid parameter, plasma hepatocyte growth factor (HGF), serum superoxide
dismutase (SOD) , malondialdehyde (MDA), Tumor necrosis factor-α (TNF-α), a Soluble intercellular adhesion molecule-1 (sICAM-1),
Lectin-like oxidized cellulose low density lipoprotein receptor-1 (LOX-1), as
well as aortic endothelial p-GSK-3β,
GSK-3β, p-Akt, Akt expressions were
determined. Results: In comparison with the control group, the model group
showed a significant increase in the levels of serum total cholesterol (TC),
triglyceride (TG), low density lipoprotein cholesterol ( LDL-C) and significant
decrease in the level of serum high density lipoprotein cholesterol (HDL-C)
after high-fat diet for 18 weeks (p α, and sICAM-1 levels were observed
relative to the control group (p β in rat aorta. Conclusion: Short-term
high-fat diet could induce vascular endothelium injury by increasing inflammation
and oxidative stress. And PI3K/Akt pathway could play an important role in hyperlipidemia-induced vascular endothelium injury.