TITLE:
Response of Explants of Calli Rice (Oryza sativa L.) Japonica cv. “Ilmi” to Gene Transformation Using Agrobacterium tumefaciens-Mediated
AUTHORS:
Fika Ayu Safitri, Mohammad Ubaidillah, Miswar , Kyung-Min Kim
KEYWORDS:
AtBI-1; Agrobacterium-Mediated; GFP; Transformation; Rice
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.4,
April
17,
2013
ABSTRACT:
The AtBI-1
gene encoding the Arabidopsis thaliana Bax inhibitor was introduced into Japonica
cultivars of rice (“Ilmi”) by Agrobacterium-mediated
transformation, and a large number of transgenic plants were produced. The
neomycin phosphotransferase II (NPTII) gene was used as a
selectable marker. The activity of neomycin phosphotransferase could be
successfully detected in transgenic rice calluses. Introduction of the AtBI-1
gene was also confirmed by PCR using AtBI-1 specific
oligonucleotide primers in regenerated plants. Stable integration
and expression of the AtbI-1 gene in plants were confirmed by GFP analysis.