TITLE:
In Vitro Sickling Inhibitory Effects and Anti-Sickle Erythrocytes Hemolysis of Dicliptera colorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench
AUTHORS:
Pius T. Mpiana, Blaise K. Lombe, Aladdin M. Ombeni, Koto-Te-Nyiwa Ngbolua, Damien S. T. Tshibangu, Louisette K. Wimba, Dorothée D. Tshilanda, Felicien K. Mushagalusa, Sévérin K. Muyisa
KEYWORDS:
Sickle Cell Disease; Antisickling Activity; Anti-Hemolytic Properties; Diclipteracolorata ; Sorghum bicolor; Euphorbia hirta; Anthocyanins
JOURNAL NAME:
Open Journal of Blood Diseases,
Vol.3 No.1,
March
29,
2013
ABSTRACT: Diclipteracolorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench are reported among plant species used in Congolese traditional medicine to treat
Sickle Cell Disease. These medicinal plants have been investigated for their
inhibitory effect on the induced sickling process of red blood cells using
Emmel’s test. Aqueous plant extracts showed good antisickling activity as
revealed by the observed normal biconcave form of sickle red blood cells in
anoxic conditions. The calculated radius of treated red blood cells by Euphorbia hirta L., Diclipteracolorata C. B. Clarke and Sorghum biocolor (L.) Moench extracts are 3.31 ± 0.55; 3.34 ± 0.53; 3.17 ± 0.52, respectively. Euphorbia hirta L. is the most active with a normalization
rate, more than 70%. The chemical screening performed on these plants showed
the presence of anthocyanins which were then extracted. The test carried out
with anthocyanin extracts showed that these phenolic compounds have a good
antisickling activity and, additionally, possess an anti-hemolytic effect on SS
blood. This suggests that the evidenced biological activity of these plants
would be due to anthocyanins. The results hence obtained
justify the use of these plants in Congolese traditional medicine.