TITLE:
Isolation and Characterization of S-Enantiomer in Montelukast
AUTHORS:
Vijaya Lakshmi Maddala, Kishore Kumar Kakumani, Kameswara Rao Chimalakonda, Srinivasulu Polisetty, P. C. Ray
KEYWORDS:
(S)-Isomer; Montelukast; Supercritical Fluid Chromatography (SFC)
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.1,
January
22,
2013
ABSTRACT:
A simple and rapid Supercritical Fluid Chromatography (SFC)
Method has been developed to isolate and characterize the S-Isomer of Montelukast
by using normal phase Amylose based AS-H with 250 mm × 30 mm, 5 microns column
using a mobile phase system containing super critical fluid carbon dioxide (CO2)
and the percentage of 2-Propanol as a mobile phase (85:15) and detection at 230
nm. The isolated S-Isomer was characterized by using FT-IR, ESI-MS, HPLC and 1H
NMR. The purity of isolated S-Isomer is about 98%.