D. W. Zhu, L. S. Wang, Y. Zhou, D. M. Li, L. M. Sun, J. F. Yang, H. T. Wu, H. Q. Zhou and M. Tada, “Antioxidant Activity of Sulphated Polysaccharide Conjugates from Abalone (Haliotis Discus Hannaiino),” European Food Research and Technology, Vol. 227, No. 6, 2008, pp. 1663-1668. doi:10.1007/s00217-008-0890-2

has been cited by the following article:

• TITLE: Antioxidant and Anti-Tyrosinase Activity from Olea Leaf Extract Depended on Seasonal Variations and Chromagraphy Treatment

  AUTHORS: Dao-Mao Yang, Ming-An Ouyang

  KEYWORDS: Olive Leaf Extract; Antioxidant Activity; Tyrosinase Inhibitory Activity; Non-CompetitiveCompetition; Chromagraphy Treatment

  JOURNAL NAME: International Journal of Organic Chemistry, Vol.2 No.4, December 28, 2012

  ABSTRACT: In the present study, olive leaf extract, harvested from four seasons, extracted with solvent (methanol and water), was treated by D101 and Sephadex LH-20, and divided into 10 fractions by TLC detection. The antioxidant activity was evaluated using DPPH, superoxide anion scavenging and reducing power assays. Tyrosinase inhibitory activity was also performed with L-DOPA as substrate. On extraction method, there is no significant difference in methanol and water. Among 12 months, autumn showed the best harvest time in whole. OLE showed 90% tyrosinase inhibitory activity and inhibition type was non-competitive competition. By chromagraphy treatment, the ingredients of polysacchmaride and protein showed high bioactivity towards antioxidant and anti-tyrosinase activity, the rest part showed different extent of activity and prove some effect by chromatography treatment indirectly. OLE might be a valuable bioactive source and can be applicable in both the cosmetic and medical use.