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has been cited by the following article:

• TITLE: Simultaneous Determination of Amlodipine with H1-Receptor Antagonists by Reversed Phase High Performance Liquid Chromatography and Application to Interaction Studies

  AUTHORS: Muhammad Saeed Arayne, Najma Sultana, Saima Sher Bahadur, Muhammad Nawaz

  KEYWORDS: Amlodipine; Cetirizine; Fexofenadine; Buclizine; Interactions; Reversed Phase High Performance Liquid Chromatography

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.3 No.9, September 28, 2012

  ABSTRACT: A rapid, fast and precise method has been developed and validated for the simultaneous determination of amlodipine with H1-receptor antagonists (cetirizine, fexofenadine, and buclizine) from dosage forms. The chromatography was performed on a Purospher? Star, C18 (5 mm, 250 × 4.6 mm) column using acetonitrile: buffer (0.01 mM) (40:60, v/v, pH adjusted to 3.0), as a mobile phase. The mobile phase was pumped at a flow rate of 1.0 mL·min-1 and UV detection was performed at 240 nm. The method was validated for linearity, accuracy, precision and specificity. The method was applied to study the interaction between amlodipine and H1-receptor antagonists. These interactions were carried out in simulated gastric juice (pH 1), simulated full stomach (pH 4), blood pH (pH 7.4) and simulating GI (pH 9). The interacting drugs were heated at 37℃ with intermit-tent shaking and the samples were withdrawn every thirty minutes for three hours and drug contents were analyzed by RP-HPLC techniques. In most cases the in vitro availability of amlodipine was decreased. It was observed that the change in in vitro availability was pH dependent.