Y. Y. Zhu, E. M. Machleder, A. Chenchik, R. Li and P. D. Siebert, “Reverse Transcriptase Template Switching A SMARTTM Approach for Full-Length cDNA Library Construction,” BioTechniques, Vol. 30, No. 4, 2001, pp. 892-897. - References

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Y. Y. Zhu, E. M. Machleder, A. Chenchik, R. Li and P. D. Siebert, “Reverse Transcriptase Template Switching: A SMARTTM Approach for Full-Length cDNA Library Construction,” BioTechniques, Vol. 30, No. 4, 2001, pp. 892-897.

has been cited by the following article:

TITLE: Effective Procedure for Development of EST-SSR Markers Using cDNA Library

AUTHORS: Kyung A Kim, Hee-Cheon Park, Jae-Keun Sohn, Kyung-Min Kim

KEYWORDS: cDNA; EST-SSR Markers; Radioactivity; Rice

JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.9, September 27, 2012

ABSTRACT: The present study was conducted to develop EST-SSR markers using the cDNA library from rice plant. Total RNA extracted from the leaves of brown plant hopper resistance gene originated from a rice cultivar “Cheongcheong” and sensitive rice cultivar “Nakdong” were used to synthesize a cDNA library. As a result of analyzing the cDNA library, the 17 EST-SSR primer sets were developed. This study enables to provide effective marker assisted selection (MAS) methods on the selection of white-backed planthopper resistance gene originated from a rice plant more simply, quickly and precisely. Furthermore, using this marker’s advantage of deriving from cDNA, it is possible to identify the white-backed planthopper resistance gene. In addition, this study introduces a technique for construction of a cDNA library safely without using radioactivity.