TITLE:
Genetic Diversity in Selected Indian Mungbean [Vigna radiata (L.) Wilczek] Cultivars Using RAPD Markers
AUTHORS:
Subhojit Datta, Sarika Gangwar, Shiv Kumar, Sanjeev Gupta, Rita Rai, Mayank Kaashyap, Pallavi Singh, Sushil Kumar Chaturvedi, Brij Bhuvan Singh, Nagaswamy Nadarajan
KEYWORDS:
Fingerprinting; Genetic Diversity; Mungbean; RAPD
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.3 No.8,
August
31,
2012
ABSTRACT: Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in Indian mungbean cultivars. A total of 60 random primers were used in the study and 33 of them generated reproducible RAPD patterns. Amplification of genomic DNA of most popular 24 Indian mungbean cultivars with these RAPD primers yielded 249 fragments that could be scored, of which 224 were polymorphic, with an average of 7.0 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPI 9) to 17 (OPD 7). Percentage polymorphism ranged from 33% (OPX 5) to a maximum of 100% (OPX 4, OPX 6, OPX 13, OPX 15, OPX 19, OPD 5, OPD 7, OPD 20, OPI 4, OPI 6, OPI 13, OPI 14, OPI 18 and OPF 1), with an average of 90%. The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis. And genotypes grouped in two major groups. Sixteen out of 24 released cultivars grouped to cluster I. This indicated the narrow genetic base in the Indian mungbean cultivars used in the study. The details of diversity analysis and possible reasons for narrow genetic base in mungbean cultivars are discussed in the present study.