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has been cited by the following article:

• TITLE: A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics

  AUTHORS: Chen Qian, Yanjuan Yuan, Xuejun He, Jing Liu, Qing Shao, Hua Wu, Hongqun Qiao

  KEYWORDS: Dencichine; (L)-2-Amino-3-(Carboxymethylthio)Propionic Acid; Hplc-Ms/Ms; Pharmacokinetic;Ammonium Acetate Solution-Acetonitrile (35:65, V/V) Mobile Phase

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.3 No.8, August 27, 2012

  ABSTRACT: In order to quantitate dencichine in biological samples, a selective and sensitive method for the determination of dencichine in rat plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. (l)-2-amino-3-(carboxymethylthio)propionic acid was used as the internal standard (I.S.). After a protein precipitation extraction with acetonitrile, dencichine and the I.S. were chromatographed on an Xterra MS-C18 column. The mobile phase was consisted of 20mmol/L ammonium acetate solution-acetonitrile (35:65, V/V) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole mass via electrospray ionization (ESI) source in the positive mode. The intra- and inter-day precision (relative standard deviation, R.S.D.) values of dencichine were below 6.7%. The extraction recoveries were up 85%. The lower limit of quantification was 20 ng/ml, which was sensitive enough to detect the analyte. The HPLC-MS/MS method was successfully applied to the pharmacokinetic study after an intravenous administration of dencichine in Sprague-Dawley (SD) rat.