Y. Uematsu, M. Ogimoto, J. Kabashima, K. Suzuki and K. Ito, “Fast Cleanup Method for the Analysis of Sudan I-IV and Para Red in Various Food and Paprika Color (Oleoresin) by High-Performance Liquid Chromatography/ Diode Array Detection: Focus on Removal of Fat and Oil as Fatty Acid Me-thyl Esters Prepared by Transesterification of Acylglycerols,” Journal of AOAC International, Vol. 90, No. 2, 2007, pp. 437-445.

has been cited by the following article:

• TITLE: Extraction Method and Determination of Sudan I Present in Sunset Yellow FCF by Isocratic High-Performance Liquid Chromatography

  AUTHORS: Chiye Tatebe, Takashi Ohtsuki, Noriko Otsuki, Hiroki Kubota, Kyoko Sato, Hiroshi Akiyama, Yoko Kawamura

  KEYWORDS: Sudan I; Sunset Yellow FCF; HPLC

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.3 No.8, August 27, 2012

  ABSTRACT: A method to extract and analyze Sudan I present in Sunset Yellow FCF (SYF) products was developed and validated. The method included the simple extraction of Sudan I from the SYF product using water, acetonitrile, and ethyl acetate and high-performance liquid chromatography (HPLC) analysis with isocratic elution using acetonitrile:water (7:3) with a photodiode array detector at 485 nm. This method was found to remove most of the excess SYF colorant and other impurities before injection to the HPLC instrument, making it easy to maintain precision control in routine laboratory tests for Sudan I in the SYF colorant. The detection limit of Sudan I in SYF products was 0.2 μg/g. A survey conducted to determine Sudan I in 13 commercial SYF samples from Japanese manufacturers from 1970 to 2010 showed that the levels of Sudan I ranged from 0.3 to 1.9 μg/g in products manufactured from 1970 to 1996 and were below the limit of detection in products manufactured after 2005.