TITLE:
Improved Solid-Phase Peptide Synthesis of Wild-Type and Phosphorylated Phospholamban Using a Pseudoproline Dipeptide
AUTHORS:
Shadi Abu-Baker, Gary A. Lorigan
KEYWORDS:
Solid-State Peptide Synthesis; Pseudoproline Dipeptide; Phospholamban
JOURNAL NAME:
Open Journal of Synthesis Theory and Applications,
Vol.1 No.2,
July
30,
2012
ABSTRACT: In this study, we report that the insertion of a pseudoproline dipeptide for the solid-phase peptide synthesis of wild-type Phospholamban protein (WT-PLB) has two important advantages. First, it disrupts the formation of different secondary structures, which is responsible for poor couplings during the preparation of highly aggregated sequences. Second, it enhances the purities and solubility of crude products leading to easier HPLC purification.