R. K. Mishra, A. Kumar, A. C. Shukla, P. Tiwari and A. Dikshit “Quantitative and Rapid Antibacterial Assay of Micromeria biflora Benth. Leaf Essential Oil against Dental Caries Causing Bacteria Using Phylogenetic Approach,” Journal of Ecobiotechnology, Vol. 2, No. 4, 2010, pp. 22-26.

has been cited by the following article:

• TITLE: Therapeutic Effects of Essential Oil from Waste Leaves of Psidium guajava L. against Cosmetic Embarrassment Using Phylogenetic Approach

  AUTHORS: Ahsan Kamran, Rohit Mishra, Rita Gupta, Awadhesh Kumar, Ashok Bajaj, Anupam Dikshit

  KEYWORDS: Essential Oil; Dermatophytes; Phylogeny; Discarded Leaves; Psidium guajava L.; Trichophyton rubrum; T. mentagrophyte

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.6, June 21, 2012

  ABSTRACT: Medicinal plants are facing threats due to loss of habitat and overexploitation of knows species. Therefore the present work shows utilization of essential oil from discarded leaves of Psidium guajava Linn. against human dermatophytic fungi Trichophyton rubrum, T. mentagrophytes, Trichophyton violaceum, Trichophyton tonsurans, Epidermophyton floccosum, Microsporum gypseum and Microsporum canis. The oil was found to be fungicidal at 3.0 μl/ml and it tolerated heavy inoculum of pathogens at fungicidal concentrations. The fungicidal activity of the oil was thermostable, up to 70°C and shelf life was found to be six months, which was maximum the time taken into consideration. The oil having cineole, caryophyllane, copaene, azulene and eucalyptol as main constituents, exhibited broad fungicidal activity. The oil did not show any adverse effect on mammalian skin upto 5% concentration. Phylogeny of the dermatophytes with respect to toxicity of the oil has also been discussed using molecular data. The cost effective formulation from waste leaves can be exploited after undergoing double blind successful multicentral topical testing.