K. K. Nkongolo, P. Michael and T. Demers, “Application of ISSR, RAPD, and Cytological Markers to the Certification of Picea mariana, P. glauca, and P. engelmannii Trees, and Their Putative Hybrids,” Genome, Vol. 48, No. 2, 2005, pp. 302-311. doi:10.1139/g04-118

has been cited by the following article:

• TITLE: Genetic Variation in Picea mariana × P. rubens Hybrid Populations Assessed with ISSR and RAPD Markers

  AUTHORS: Ramya Narendrula, Kabwe Nkongolo

  KEYWORDS: Picea mariana; Picea rubens; Interspecific Hybrids; Inter Simple Sequence Repeat; Random Amplified Polymorphic DNA

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.6, June 21, 2012

  ABSTRACT: Interspecific hybridization can result in significant shifts in allele frequencies. The objective of the present study was to assess the level of genetic variation in populations of P. mariana × P. rubens hybrids derived from artificial crosses. Progenies from backcross populations created through a series of controlled pollinations among P. mariana and P. rubens trees across the hybridization index were analyzed. Several Inter Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) primers were used to amplify genomic DNA samples from each population. ISSR primers produced from 30% to 52% polymorphic loci. The level of polymorphism was higher with RAPD markers, ranging from 57% to 76%. Overall, the two marker systems generated similar levels of polymorphic loci for P. mariana and P. rubens populations. No significant differences were found among the P. mariana × P. rubens populations analyzed and between the hybrids and the parental populations regardless of the molecular marker used. This confirms the genetic closeness of P. mariana and P. rubens species.