K. Kuypers, P. Cos, E. Ortega-Barria, D. Vanden Berghe and L. Maes, Bioassays for Some Parasitic Protozoa, Screening Concepts and Standard in Vitro and in Vivo Laboratory Model,” In: M. P. Gupta, S. S. Handa and K. Vanish, Eds., Biological Screening of Plant Constituents, International Centre for Science and High Technology, Trieste, 2006, pp. 7-18.

has been cited by the following article:

• TITLE: In Vitro Antiprotozoal and Cytotoxic Activity of the Aqueous Extract, the 80% Methanol Extract and Its Fractions from the Seeds of Brucea sumatrana Roxb. (Simaroubaceae) Growing in Democratic Republic of Congo

  AUTHORS: Michel Tshodi Ehata, Adelard Mbenza Phuati, Stanislas Nsaka Lumpu, Cyprien Kikweta Munduki, Donatien Bakana Phongi, Gaston Tona Lutete, Oscar Kambu Kabangu, Richard Cimanga Kanyanga, Ann Matheeussen, Paul Cos, Sandra Apers, Luc Pieters, Louis Maes, Arnold J. Vlietinck

  KEYWORDS: Brucea sumatrana; Simaroubaceae; Seeds; Antiprotozoal Activity; Cytotoxicity

  JOURNAL NAME: Chinese Medicine, Vol.3 No.1, March 27, 2012

  ABSTRACT: The in vitro antiprotozoal and cytotoxic activity of the aqueous extract, the 80% methanol extract, and its different soluble fractions and subfractions from Brucea sumatrana seeds were assessed against two Trypanosoma (T. cruzi and T. brucei brucei), Leishmania infantum and chloroquine and pyrimethanine-resistant K1strain of P. falciparum and against MRC-5 cell-lines respectively. Results indicated that the 80% methanol extract showed a cytotoxic effect against MRC-5 cell lines with CC50 value of 0.54 μg/ml. It however exhibited pronounced and non selective activity against T. cruzi (IC50 = 1.52 μg/ml, SI = 0.03) and L. infantum (IC50 = 2.41 μg/ml, SI = 0.22). It however displayed pronounced and selective effect against T. brucei brucei (IC50 2.16) and chloroquine and pyrimethamine-resistant K1 strain of P. falciparum (IC50 2.16). All soluble fractions and subfractions from the partition of the 80% methanol extract were found to exhibit an antiprotozoal activity with IC50 values ranging from T .cruzi, T. b. brucei, L. infantum and chloroquine and pyrimethamine-resistant K1 strain of P. falciparum with IC50 values of 0.33, 81, 81 and >81 respectively. The chloroform soluble fraction rich in alkaloid was cytotoxic against MRC-5 cell lines (CC50 = 27.09 μg/ml) and showed good activity against T. b. brucei (IC50 = 8.36 and SI = 3.24) and moderate activity against T. cruzi, L. infantum and chloroquine-pyrimethane-resistant K1 strain of P. falciparum (20 50 50 = 1.55 and 0.43 μg/ml respectively), they however displayed pronounced antiprotozoal activity against T. cruzi, T. b. brucei and chloroquine and pyrimethamine-resistant K1 strain of P. falciparum with IC50 values ranging from P. falciparum (SI = >6.2 and >1.72 respectively). These extracts however showed good and low activity respectively against L. infantum (IC50 = 24.05 and 6.82 μg/ml respectively).