TITLE:
Isolation and Authentication of Local Rhizobia Nodulating Common Bean (Phaseolus vulgaris L.) in Different Agro-Ecological Zones of Côte d’Ivoire
AUTHORS:
Marie-Paule Aboueuh Atsé, Mako François De Paul N’Gbesso, Ibrahim Konaté, Noupé Diakaria Coulibaly, Kouadio Amani, Katiénapariga Tayourou Yéo, Louis Butare
KEYWORDS:
Trapping, Native Rhizobia, Nodulation, Common Bean, Côte d’Ivoire
JOURNAL NAME:
Advances in Microbiology,
Vol.13 No.9,
September
8,
2023
ABSTRACT: Legumes such as common bean (Phaseolus vulgaris L.) have been introduced into cropping systems for sustainable soil management. Consequently, the loss of fertility of the latter remains a major constraint to bean production because this legume is rarely fertilized, yet it is considered to be a poor nitrogen fixer in the absence of inoculation. To overcome this, this study was undertaken with the objective of seeking efficient local rhizobia in order to propose a bean inoculum formulation. To do this, soil samples taken from twelve localities in the Centre, North and West areas of C?te d’Ivoire were used to trap bean nodulating rhizobia. The ROBA1 bean accession used was sown in pots containing the sampled soils. Seedlings were uprooted at the start of flowering and nodulation was assessed. The isolates obtained were purified and then characterized phenotypically. The infectivity and symbiotic efficacy of these isolates were determined in vitro by the authentication test in which the purified isolates were reinoculated to their original host plant. A total of 24 rhizobium isolates were obtained from the soils of six localities. During morphological characterization, the isolates showed typical characteristics of Rhizobium. With the exception of RPC501, RPC505 and RPC522, all isolates were authenticated and able to nodulate the host plant in controlled culture. Isolates RPC502, RPC507, and RPC508 were effective and significantly increased (P