TITLE:
Semi-Automated Enzymatic Determination of Ethanol in Beverages: Collaborative Study for RIDA®CUBE Ethanol
AUTHORS:
Markus Lacorn, Thomas Hektor
KEYWORDS:
Ethanol, Enzymatic Analysis, Beverages, Automation, Reproducibility, Collaborative Test
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.14 No.2,
February
24,
2023
ABSTRACT: Easy and quick methods to quantify ethanol reliably in beverages are
always important. In 2022, the EnzytecTM Liquid Ethanol test kit was approved as AOAC Official MethodSM 2017.07 Final Action after a
collaborative study was conducted with different beverages such as kombucha,
juices, and beer. During set-up of this collaborative test, small sized
companies asked to include the RIDA®CUBE Ethanol/RIDA®CUBE SCAN device since it is easy to use, suitable
for a few samples only and contains the identical reagents as the EnzytecTM Liquid system. It is applicable to quantify ethanol in diluted kombucha, fruit juices, and alcohol-free beer samples around 0.5%
alcohol-by-volume within 12 min. The overall relative reproducibility standard
deviation across a wide concentration range for kombucha, was calculated to be 6.29%.
Analysis of juices and beer showed an overall higher variation with an
estimated overall RSD(R) value by regression of 14.4%. The data obtained by
this collaborative study show that the RIDA®CUBE Ethanol in combination with the RIDA®CUBE SCAN device is suitable to quantify ethanol from matrices representing
important alcohol-free liquid food categories.