TITLE:
DC-SIGN (CD209) Promoter -336 A/G Polymorphism Is Not Associated with Dengue Fever at Burkina Faso, West Africa
AUTHORS:
Fiffou Yougbare, Aziz Sidi Aristide Tapsoba, Pegdwendé Abel Sorgho, Bagora Bayala, Lassina Traore, Prosper Bado, Bapio Valérie Elvira Jean Télesphore Bazie, Abdou Azaque Zoure, Esther Mah Alima Traore, Théodora Mahoukèdè Zohoncon, Sessi Frida Tovo, Albert Théophane Yonli, Alice Kiba, Florencia Wendkuuni Djigma, Jacques Simpore
KEYWORDS:
Dengue Virus, CD209 Variant (-336 A/G, rs4804803), Burkina Faso
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.11 No.1,
January
31,
2023
ABSTRACT: Objective: The aim of this study was to characterize the polymorphisms of the DC-SIGN (-336 A/G, rs4804803) gene and their association with the immunopathogenicity of dengue fever in Burkina Faso. Methods: A total of three hundred forty-one subjects, patients of all ages have been included in the study: 208 persons presenting clinical signs of dengue fever which were confirmed by diagnostic and 133 Healthy Controls. Genotyping for the CD209 variant (-336 A/G, rs4804803) was carried out using TaqMan SNP Genotyping Assays. Haplotype frequencies were inferred and compared between the study groups. Results: The percentage of men was 61.88% (211/341) and 38.12% (130/341) for women. The highest frequency of dengue fever (77.42%) was noted in patients with age between 20 to 40 years. Around 1.52% of the study population was positive for HIV, 40.55% were carriers of HBV and 3.83% of HCV. Genotype distribution of the CD209 variant (-336 A/G, rs4804803) was in Hardy-Weinberg equilibrium in both patients and controls. The frequency of allele A was higher than allele G; however, statistical analyses showed that there is no significant difference in genotypes GG, AG and AA in patients and controls. Conclusion: This related no significant association with dengue for the variant of ?336 A/G in the DC-SIGN gene in an Ouagadougou population. However, our results offered the SNP frequencies in a West African population, which might be useful for the study of ethnic groups.