TITLE:
Impact of Presence or Absence of Trehalose during Vitrification on Viability and Development of Vitrified/Warmed Immature Dromedary Camel Oocytes
AUTHORS:
Karim A. Yaqout, Ahmed Monir, Magdy R. Badr, Abou Bakr A. EL-Wishy, Adel R. Moawad, Amr S. El-Shalofy
KEYWORDS:
Camel, GV, Oocyte, IVM, Trehalose, Vitrification
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.12 No.3,
June
16,
2022
ABSTRACT: Vitrification
of immature oocytes at the germinal vesicle (GV) stage is important to preserve
female gametes. The standard formula for vitrification solutions has long been
a debate. Herein, we investigated the effect of the presence or absence of trehalose in vitrification
solution on viability, in vitro maturation (IVM) rates, and development of
vitrified/warmed immature dromedary camel
oocytes. Cumulus oocyte complexes (COCs) obtained at slaughter from the
ovaries of mature she-camels were randomly allocated into three groups; namely,
control group, oocytes were directly
subjected to IVM without vitrification, vitrification solution 1 (VS1) group, oocytes were vitrified in a solution composed of 25% ethylene glycol (EG) plus 25% dimethyl
sulfoxide (DMSO) + 0.5 M trehalose; and vitrification solution 2 (VS2) group, oocytes were vitrified in a solution composed of 25%
EG plus 25% DMSO. Vitrification of COCs was conducted by open pulled straws
(OPS) method. Following vitrification and warming, morphologically viable
oocytes were matured in vitro for 36 h. COCs were then fertilized and cultured in vitro for 7 days. The percentage of
viable oocytes was significantly higher (P 0.05) in VS2 than VS1 group (80.0% vs. 63.3%, respectively). Nuclear maturation, cleavage (48 h
post-insemination; pi), and blastocyst rates (7 days pi) were significantly higher (P camel oocytes in trehalose-free
solution (VS2) was more advantageous than that in trehalose supplemented media
since it did not reduce viability and development.