TITLE:
Phytochemical Study and Evaluation of the Antiradical Activity of Extracts of Oleaginous Seeds of Panda oleosa and Isolona hexaloba from Gabon
AUTHORS:
M.-A. N’negue ép Mezui-Mbeng, Darina Medza, Prosper Edou Engonga, Ognane Bikoro Lié, Lyne Mengome, Nestor Engone, Abougone Sophie
KEYWORDS:
Panda oleosa, Isolona hexaloba, Oleaginous Seeds, Extracts, Phytochemical Screening, Antiradical Activity, ABTS-+, Gallic Acid
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.13 No.2,
February
17,
2022
ABSTRACT: Our study focused on phytochemical tests
and evaluation of the anti-free radical activity of seed extracts of two
oleaginous plants from Gabon used in traditional medicine or as condiments: Panda oleosa and Isolona hexaloba. The
extraction was carried out by maceration with solvents of increasing polarity:
cyclohexane, trichloroethylene, acetone, ethanol and finally distilled water.
The total yields of the extracts are about 69.50% for Panda oleosa and 34.28% for Isolona hexaloba. The
phytochemical tests carried out on the extracts of the seeds of Panda and
Isolona highlight in both seeds the presence of alkaloids, polyphenols,
triterpenes, carotenoids, reducing compounds, flavonoids, total sugars,
coumarins, anthraquinones, free quinones, free anthracene derivatives, and
terpenoids. Isolona seeds also contain leucoanthocyanins, sterols, cardiac
glycosides and saponins. Phytochemical tests revealed the absence of tannins
and mucilage in both seeds. The free radical scavenging activity was measured
by scavenging the free radical cation of 2,2’-azino-bis[3-ethylbenzothiazoline-6-sulfonic
acid] (ABTS-+) with gallic acid as the reference antioxidant. The results of
the free radical scavenging activity of the aqueous and ethanolic extracts of
both seeds showed that the aqueous extracts were more active than the ethanolic
extracts. The IC50s of the aqueous and ethanolic extracts of Panda seeds are 40
and 60 μg·mL-1 respectively, and those of the aqueous and ethanolic
extracts of Isolona are 37.5 and 95 μg·mL-1 respectively. Gallic
acid, the reference antioxidant (IC50 = 0.37 μg·mL-1) is about 10
times more active than the aqueous extracts of both seeds, 16 times more active
than the ethanolic extract of Panda and 25 times more active than the ethanolic
extract of Isolena.