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has been cited by the following article:

• TITLE: Insulin Effect on Gene Expression of Dental Pulp Cell during Osteodifferentiation

  AUTHORS: Fidan Huseynova, Alamdar Mammadov, Irada Huseynova, Frédéric Cuisinier, Véronique Barragan-Montero

  KEYWORDS: Dental Pulp Cells, Gene Expression, Osteodifferentiation, Insulin

  JOURNAL NAME: Journal of Biosciences and Medicines, Vol.10 No.1, January 24, 2022

  ABSTRACT: Insulin, an old but still actual molecule, has many roles in various cellular processes including bone formation and also angiogenesis. Insulin effects on different cell types were investigated, and we intended to check its effect on dental pulp cells (DPC) during osteoblast differentiation. First, bone differentiation ability of isolated dental pulp cells was assessed by alizarin red (AR) staining. Insulin increased dental pulp cell proliferation after 25 hours of culture. It increased mRNA expression of osteogenic markers such as Col1, RunX2, ALP, Osc, Mef2C and expression of genes involved in TGF b pathway such as Smad3, TSP1, VEGF at different time points.