TITLE:
Effects of Supplemental Feeding of Probiotics during Lactation on Rumen Microflora of Calves after Weaning
AUTHORS:
Xueyan Lin, Tian Zhang, Lin Ju, Yue Jiang, Qiuling Hou, Zhiyong Hu, Yun Wang, Zhonghua Wang
KEYWORDS:
Calves, Probiotics, Rumen Fluid, Serum, Metabolites
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.12 No.7,
July
22,
2021
ABSTRACT: For the experiment, 8 newborn male Holstein calves
were selected that had the same feeding environment, and were of similar ages.
They were randomly divided into 2 groups,
with 4 in each group. The treatments consisted of feeding active
probiotics (Group P) and a normal fed control group (Group C). The growth
performance and blood indices were measured; rumen fluid samples were collected
after weaning, and 16SrDNA sequencing and LC-MS metabolome detection were
performed. Compared with the control group, relative
abundances of Deltaproteobacteria, Desulfovibrionales, Bacteroidales_ BS11_gut_group, Desulfovibrionaceae,
Bacteroidales_S24-7_group, Acetobacteraceae,
Ruminococcaceae_NK4A214_group, Asaia, [Ruminococcus] gauvreauii_group, Desulfovibrio, Kingella, Selenomonas,
Lachnoclostridium in group P were
significantly different (P of 2-methylbenzoic acid and myo-inositol were
significantly increased (P 0.05). These results showed that
compared with normally fed calves, the growth performance and blood indices of
probiotic-fed calves were changed, but the differences were not significant.
Probiotic-fed calves showed significant differences in rumen fluid and a small
number of metabolites, which were mainly involved in the pathway of
carbohydrate metabolism. It proves that the
supplemental active probiotics had an effect on the rumen microflora.