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has been cited by the following article:

• TITLE: Comparison of Two Enzyme Immunoassays and Four Lysate Antigens for the Detection of Antibody in Canine Blastomycosis

  AUTHORS: Matthew Day, Arin Esterbrook, Ignatius Bisharat, Abdullah Saleh Albqomi, Bryn Kennell, Russ Manteca, Heaton Oakes, Geoffrey M. Scalarone, Gene M. Scalarone

  KEYWORDS: Blastomyces lysate Antigens, Antibody Detection, ELISA Methods, Canine Blastomycosis, Blastomyces dermatitidis, Blastomyces gilchristii

  JOURNAL NAME: Open Journal of Veterinary Medicine, Vol.11 No.4, April 28, 2021

  ABSTRACT: Blastomycosis, the systemic fungal disease of humans and animals caused by Blastomyces dermatitidis and the cryptic species Blastomyces gilchristii, is often misdiagnosed as a bacterial or viral pulmonary disease. Therefore, the development of improved immunodiagnostic assays for this disease has been the primary focus of research in our laboratory. The present study was designed to evaluate four Blastomyces yeast-phase lysate antigenic preparations (human, 597, Eagle River, WI; dog, ERC-2, WI; Human, B5927, Mountain Iron, MN; soil, 85, Georgia, ATCC 56920) for their ability to detect antibody in 48 serum specimens from dogs with diagnosed blastomycosis using an indirect ELISA (STD) compared to a biotin-streptavidin ELISA (B-SA). All four lysate antigens were able to detect antibodies in the specimens with mean absorbance values ranging from 0.930 (B5927) to 1.142 (ERC-2) with the STD ELSA and from 1.395 (B5927) to 1.775 (85) with the B-SA ELISA. The results indicated that both ELISA methods could be utilized for antibody detection, but the B-SA ELISA exhibited greater sensitivity than the STD ELISA with all four of the lysates.