Glembotski, C.C., Thuerauf, D.J., Huang, C.Q., Vekich, J.A., Gottlieb, R.A. and Doroudgar, S. (2012) Mesencephalic Astrocyte-Derived Neurotrophic Factor Protects the Heart from Ischemic Damage and Is Selectively Secreted upon Sarco/Endoplasmic Reticulum Calcium Depletion. The Journal of Biological Chemistry, 287, 25893-5904. - References

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Glembotski, C.C., Thuerauf, D.J., Huang, C.Q., Vekich, J.A., Gottlieb, R.A. and Doroudgar, S. (2012) Mesencephalic Astrocyte-Derived Neurotrophic Factor Protects the Heart from Ischemic Damage and Is Selectively Secreted upon Sarco/Endoplasmic Reticulum Calcium Depletion. The Journal of Biological Chemistry, 287, 25893-5904.
https://doi.org/10.1074/jbc.M112.356345

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AUTHORS: Swati Sharma, Gurudutta U. Gangenahalli, Upma Singh

KEYWORDS: Hematopoietic Stem Cells (HSCs), Signal Transduction, Haematopoiesis

JOURNAL NAME: CellBio, Vol.9 No.3, September 30, 2020

ABSTRACT: Hematopoietic stem cells (HSCs) are tissue-specific cells giving rise to all mature blood cell types regulated by a diverse group of hematopoietic cytokines and growth factors that influences the survival & proliferation of early progenitors and differentiation mechanisms by modulating the functional activities of HSCs. In this study, the functional yet distinctive role of three novel combinations of gene pairs RRAGC & PSMC2; CKAP4 & MANF; and CTR9 & CNTNAP2 have been newly identified. These novel combinations of genes were confirmed and expressed in K562 human leukemic cell line in the presence of cytokine combination (IL-3, FLT-3 and SCF) using RT-PCR and siRNA-mediated gene knock down strategy. This study signifies the synergistic role of gene pairs in different molecular activities like ubiquitination or proteasomal degradation, calcium mobilization, dopamine signaling.

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