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has been cited by the following article:

• TITLE: Development of α-Amino Acid Detection with Peroxyoxalate Chemiluminescence by Using Water-Acetonitrile-Ethyl Acetate Mixed Solution

  AUTHORS: Hyo Kan, Ryo Mizutani, Kazuhiko Tsukagoshi

  KEYWORDS: Peroxyoxalate chemiluminescence, α-Amino Acids, Water-Acetonitrile-Ethyl Acetate, Bis(2, 4, 6-Trichlorophenyl) Oxalate

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.11 No.1, January 21, 2020

  ABSTRACT: Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the ternary solutions of water (1.0 mM carbonate buffer, pH 9.0)-acetonitrile-ethyl acetate, water-rich of 15:3:2 volume ratio and organic solvent-rich of 3:8:4 volume ratio, to which bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide chemiluminescence reagent were added. The chemiluminescence observed with the ternary solutions, especially the organic solvent-rich solution, showed a larger signal than that observed with the water only solution or water-acetonitrile mixed solution. Chemiluminescence in the presence of twenty types of α-amino acid was similarly examined by using the ternary organic solvent-rich solution. The chemiluminescence of three α-amino acids with fluorescence properties was enhanced with the ternary solution. The data reported here may contribute to development of a new, sensitive peroxyoxalate chemiluminescence detection system.