TITLE:
Effect of Different Inducers on the Essential Oil of Suspension Culture Cells from Cinnamomum longepaniculatum
AUTHORS:
Linman He, Kuan Yan, Mingbo Xiao
KEYWORDS:
Cinnamomum longepaniculatum, Suspension Cells, Single Factor Orthogonal Test
JOURNAL NAME:
Open Access Library Journal,
Vol.6 No.10,
October
15,
2019
ABSTRACT: In this study, the effects of different inducers on the accumulation of essential oil (1,8-eucalyptus) in suspension cells of Cinnamomum longepaniculatum were studied by adding different inducers, and the differences in the effects of different inducers on the yield and quality of essential oil in suspension cells of C. longepaniculatum were revealed. The results showed that under the same conditions, the addition of CaSO4 1.5 mg/L, Li2SO4 1.0 mg/L, SA15 mg/L and H2O2 9 mmol/L in B5 medium could significantly promote the accumulation of essential oil, which was 60.94%, 54.69%, 36.72% and 35.16% higher than that of CK, reaching 0.0824 mg/L, 0.0792 mg/L, 0.0700 mg/L and 0.0692 mg/L, respectively. Through orthogonal test, it was found that 2.0 mg/L CaSO4 combined with, H2O2 9 mmol/L, SA 15 mg/L and Li2SO4 0.5 mg /L presented significant differences compared with other combinations (P