Kumar, P.M., Suba, V., Reddy, R.B. and Babu, S.P. (2017) Inhibitory Effects of Oncoba spinosa on Key Enzymes Related to Diabetes Mellitus (α-Amylase and α-Glucosidase) and Obesity (Pancreatic Lipase) in Vitro. Diabetes & Metabolism, 8, 781.
https://doi.org/10.4172/2155-6156.1000781
-
TITLE:
Tephrosia purpurea Fraction Attenuates Lipid Accumulation and Adipogenesis in 3T3-L1 Adipocytes and Reduces Body Weight in High Fat Diet Induced Obese Rats
AUTHORS:
Karunakaran Reddy Sankaran, Lokanatha Oruganti, Krishna Chintakuntla, Balaji Meriga
KEYWORDS:
Body Weight, Cell Viability, Enzyme Inhibition, Lipolysis, 3T3-L1 Cells
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.9,
September
30,
2019
ABSTRACT: The anti-adipogenic and anti-obesity activity of
chloroform fraction of Tephrosia purpurea (CFTp) on 3T3-L1 adipocytes and high fat diet
(HFD)-fed obese rats was evaluated in this study. A substantial and dose
dependent inhibition of α-glucosidase
(81%) and lipase (75%) activities by CFTp was noticed. Treatment with CFTp (250
μg/mL) significantly inhibited 3T3-L1 adipocytes differentiation and lipid
accumulation. A semi-quantitative
RT-PCR analysis of 3T3-L1 cells revealed down regulation of mRNA expression of
peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS)
and acetyl CoA carboxylase-2 (ACC-2), while
glucose transporter type-4 (GLUT-4) expression was up-regulated in a
dose dependent manner with CFTp. Further, oral administration of CFTp (200
mg/kg.b.wt.) significantly reduced body weight gain, fat mass, blood glucose and
leptin levels in high fat diet (HFD)-induced obese rats. Taken together, these
findings demonstrate that CFTp possesses potent anti-obesity activities.
Related Articles:
Long Lasting Effects of Breastfeeding on Metabolism in Women with Prior Gestational Diabetes
Luca Mattei, Antonietta Colatrella, Olimpia Bitterman, Paola Bianchi, Chiara Giuliani, Giona Roma, Camilla Festa, Gianluca Merola, Vincenzo Toscano, Angela Napoli
DOI:
10.4236/jdm.2014.44037
3,888 Downloads 4,834 Views Citations
Pub. Date:
October
16,
2014
