TITLE:
New Method for Isolation of Naringin Compound from Citrus maxima
AUTHORS:
Aliefman Hakim, Jamaluddin , I. Nyoman Loka, Sukib , Ni Wayan Wida Sasmining Prastiwi
KEYWORDS:
Isolation, Naringin, Citrus maxima
JOURNAL NAME:
Natural Resources,
Vol.10 No.8,
August
22,
2019
ABSTRACT: This research aims to know the proper isolation method to isolate naringin compound from Citrus maxima for natural product chemistry laboratory. This natural product chemistry laboratory provides students with the essential skills and knowledge required to perform the extraction, isolation, and structural identification. The isolation began with sample preparation, extraction with methanol, partition with n-hexane, purification by crystallization using isopropanol, by analyzing the purity with Thin Layer Chromatography (TLC) tested, and the presence of functional groups with Fourier Transform Infrared (FT-IR) spectrophotometer test. The result of FT-IR spectrophotometer analysis contains hydroxy group (-OH), aromatic cyclic groups (C=C), CH2, CH3, and ether groups.