TITLE:
Identification of QTLs for Blast, Bacterial Blight, and Planthopper Resistance Using SNP-Based Linkage Maps from Two Recombinant Inbred Rice Lines
AUTHORS:
Jirapong Jairin, Phanchita Vejchasarn, Thanapa Somjai, Kanuengnij Srivilai, Kulchana Darwell, Phikul Leelagud, Rungnapa Kawichai, Jate Kotcharerk, Arissara Suthanthangjai, Nattaya Popa, Suphalaksana Lachanthuek, Varapong Chamarerk
KEYWORDS:
Recombinant Inbred Line, Genotyping by Sequencing, Resistance Gene, Rainfed Lowland Rice, Single Nucleotide Polymorphism, Quantitative Trait Locus
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.10 No.5,
May
27,
2019
ABSTRACT: Rice is the most significant global food security.
Several biotic factors limit rice production, breeding biotic-resistant rice
has, therefore, become an increasingly important goal. Two elite rice lines,
IR71033-121-15 (IR71033) and IR57514-PMI-5-B-1-2 (IR57514), provide potential
genes for biotic stress resistance traits. In this study, genotyping by
sequencing (GBS) for single nucleotide polymorphism (SNP)-based linkage map
construction was used to detect quantitative trait loci (QTLs) for blast (BL),
bacterial blight (BB), whitebacked
planthopper (WBPH), and brown planthopper (BPH) resistance. IR71033 was derived
from Oryza minuta and carried BL, BB, WBPH, and BPH
resistance QTLs. IR57514 is a well-adapted rainfed lowland line that carries BL
and BB resistance QTLs. Two sets of recombinant inbred line (RIL) populations
derived from crosses of KDML105 × IR71033 and KDML105 × IR57514 were used to
dissect the genetic basis of disease and insect pest resistance. The RIL
populations were evaluated for BL, BB, WBPH, and BPH resistance from 2016 to
2018 at four rice research centers in Thailand. From these, we identified a
large number of SNPs through GBS and constructed high-resolution linkage maps.
By combining phenotypic evaluation with the GBS data, a total of 24 QTLs on
four chromosomes were detected that confered pest
resistance and explained 7.3% - 61.4% of the phenotypic variance. These findings should
facilitate identifying novel resistance genes and applying marker-assisted
selection for resistance to the four major rice pests investigated here. These
strategies will improve the resilience and reliability of rice varieties
adapted to the low-yielding environment of rainfed lowland areas worldwide.