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has been cited by the following article:

• TITLE: Characterization of Angiotensin-Converting Enzyme before and after Cryopreservation of Gir Semen

  AUTHORS: Fernando H. G. Furtado, Fábio J. C. Faria, Elisvânia F. Santos, Ricardo G. Almeida, Deiler S. Costa

  KEYWORDS: Bovine, Spermatozoid, Seminal Proteomics

  JOURNAL NAME: Agricultural Sciences, Vol.10 No.5, May 16, 2019

  ABSTRACT: The aim of this study was to characterize the angiotensin-converting enzyme (ACE) in Gir semen before and after cryopreservation. The ejaculate of five sexually mature bulls was used. After collection, one 1-mL aliquot of fresh semen was analyzed immediately, and the rest of the semen was cryopreserved in liquid nitrogen for subsequent analysis. Freshly collected semen and thawed cryopreserved semen were centrifuged twice with Tyrode’s albumin lactate pyruvate medium (TALP) to remove plasma and extender, respectively. Samples were then subjected to western blotting, immunocytochemistry, and enzymatic activity techniques. At least one 100 kDa band was observed in every bull analyzed using western blotting with an anti-ACE monoclonal antibody, and band intensity decreased by 70% (p