TITLE:
Simple and Selective Colorimetric Detection of Oxytetracycline Based on Fe(III) Ion-3,3’,5,5’-Tetramethylbenzidine
AUTHORS:
Wenshu Yang, Zhigang Chen, Huaming Li
KEYWORDS:
Colorimetric Detection, Oxytetracycline, Oxidation, Sensor
JOURNAL NAME:
Materials Sciences and Applications,
Vol.9 No.13,
December
21,
2018
ABSTRACT: Oxytetracycline (OTC) is a common antibacterial
agent used for the control of animal diseases. OTC abuse can seriously affect
human health. Herein, based on the Fe(III)-3,3’,5,5’-tetramethylbenzidine
(Fe(III)-TMB) system, a facile and rapid colorimetricassay for oxytetracycline
(OTC) was successfully developed. The
addition of OTC could remarkably enhance the Fe(III)-oxidized TMB
reaction and the absorbance increase of Fe(III)-TMB solution is proportional to
the added OTC. The linear range of proposed sensor for OTC was from 20 nM to
1000 nM with the detection limit of 7.97 nM. The high sensitivity for OTC
detection was successfully achieved under optimal conditions. For real sample
analysis, recoveries of 89.93% to 100.02% was obtained. This is the first
report for detecting OTC based on the nonenzymatic colorimetric reaction using
the intrinsic oxidized activity of OTC/Fe3+ complex. The present
simple, low-cost and visualized sensor has great potential for OTC detection in
food.