TITLE:
Cloning and Characterization of a Mitogen-Activated Protein Kinase Gene 84KMPK14 in Hybrid Poplar (Populus alba × P. glandulosa cv. “84K”)
AUTHORS:
Ye Zhao, Dan Wang, Yongqiang Zhang, Yajie Niu, Xiaojuan Zong, Yan Ma, Xianfeng Guo, Jing Guo
KEYWORDS:
MAPK, Expression Analysis, Abiotic Stress, 84K Poplar, MPK14
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.9 No.13,
December
5,
2018
ABSTRACT: Mitogen-activated
protein kinases (MAPKs) are important components in signal transduction modules
which play crucial roles in regulation of many biological processes in plants.
Although genome-wide analysis of MAPK and MAPKK family has been carried out in
poplar species, few data about the biological function analysis of this gene
family are available to date. In this study, a group C MAPK
gene 84KMPK14 was
cloned from hybrid poplar (Populus alba × P.
glandulosa cv. “84K”). It
contained a typical protein kinase domain, a conserved TEY-motif and an atypical
conserved common docking (CD) domain. Sequence alignment revealed that 84KMPK14 was the most homologous to Populus
trichocarpa PtMPK14. Expression
analysis indicated that the transcript of 84KMPK14 in roots
and young leaves was higher than that in other tissues. The expression of 84KMPK14 was down-regulated by
low or high temperature and was induced by H2O2 significantly.
It was suppressed by drought and salinity stresses slightly one hour after
treatment and then increased quickly three hours after treatment. These results
indicated that 84KMPK14 may be involved in environmental
stresses, which provides basis for further characterization of the
physiological analysis on this gene.