TITLE:
The Seed Water Sorption Isotherm and Antioxidant-Defensive Mechanisms of Hordeum vulgare L. Primed Seeds
AUTHORS:
Pinpinatt Junhaeng, Pitipong Thobunluepop, Wanchai Chanprasert, Damrongvudhi Onwimol, Sutkhet Nakasathien, Elke Pawelzik
KEYWORDS:
Seed Moisture Sorption Characteristic, Barley Seed, Hydro-Priming, Seed Germination, Speed of Germination, Storability, Defenses Mechanism
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.9 No.12,
November
9,
2018
ABSTRACT: The key successful for
seed priming technique was the management of seed imbibition process. This
study was aimed to indicate the seed moisture sorption characteristics of Hordeum
vulgare L. cv. Samerng 2 in relation to the seed priming technique
and its effect on the seed qualities. The experiment was designed by Completely Randomized Design (CRD)
with four replications. Seed hydro-priming technique was done under 25 degree
Celsius, and 40% - 60% RH.
Barley seed moisture content was determined every hour after imbibition for 24
hours. Then, primed barley seed qualities were evaluated at 6, 8, 10, 12, 14,
and 16 hours of hydrotime. The results revealed that barley seeds were imbibed rapidly from 10.55% to 37.69% seed moisture
content during 0 - 9 hours of imbibition. The constantly seed moisture content
was continued until 17 hours of imbibition. Radicle was protruded with 42.74%
seed moisture content. Seed moisture sorption characteristic equation was Y = 0.0055x3- 0.267x2 +
4.5931x + 13.348. Coefficient of determination (R2) was
0.916. Primed seed germination was not affected by hydrotime. Primed seeds were
improved in speed of germination and seedling growth rate. The best primed
barley seed vigorous was noted
at 14 - 16 hours of hydrotime. In addition, catalase activity (CAT) and
superoxide dismutase activity (SOD) were not significantly different for
non-primed and 14 - 16
hours primed seeds. However, lipoxygenase activity (LOX) was increased and
malondialdehyde (MDA) content was decreased for 14 - 16 hours primed seeds.
Subsequently, 14 - 16
hours primed barley seeds can maintain high seed germination and vigor for 8 - 12
months under 5 degree Celsius. Thus, it could be concluded that hydro-priming
for 14 - 16
hours enhances barley
seed quality by reducing germination time, induced defenses mechanism, and
could be stored for 8 - 12 months.