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has been cited by the following article:

• TITLE: Cloning and Expression of One Anthocyanin-Related R2R3-MYB Gene in Rosa rugosa

  AUTHORS: Yang Wang, Xiaoming Sui, Mingyuan Zhao, Xu Han, Lanyong Zhao, Zongda Xu

  KEYWORDS: Rosa rugosa, Anthocyanin, R2R3-MYB, Gene Expression

  JOURNAL NAME: American Journal of Plant Sciences, Vol.9 No.10, September 17, 2018

  ABSTRACT: Based on the transcriptome of Rosa rugosa, one anthocyanin-promoting R2R3-MYB gene, RrMYB10.1 (Accession Nos:MH717244), was cloned from the petals of Rosa rugosa ‘Zizhi’. Sequence analysis results showed that RrMYB10.1 had a full length opening reading frame of 747bp, encoding 249 amino acids. Sequence analysis revealed that RrMYB10.1 contained the conserved R2R3-MYB domain, two atypical anthocyanin-promoting motifs and a conserved amino acid signature for the interaction with bHLH protein. The results of phylogenic tree revealed that RrMYB10.1 showed high homology with other anthocyanin-promoting proteins in Rosacea, and sharing the highest identity (98.39%) with RhMYB10. RT-PCR results showed that RrMYB10.1 was mainly expressed in petals among various tissues and expressed significantly higher in petals in bud stage than in opening period. To sum up, these results showed that RrMYN10.1 may play a key role in regulating anthocyanin concentration, thus providing a certain foundation on regulating flower color formation in Rosa rugosa.