TITLE:
Chemical Composition, Proapoptotic and Antiosteoporosis Activities of the Essential Oil from the Aerial Part of Alpinia officinarum Hance
AUTHORS:
Yang Wang, Ji Chen, Keda Zhang, Weigang Wu, Lanlan Ge, Haoqiang Wan, Jiemei Li, Jun Tian, Xiaobin Zeng
KEYWORDS:
Alpinia officinarum Hance, Essential Oil, Cancer, Apoptosis, Anti-Osteoporosis
JOURNAL NAME:
Chinese Medicine,
Vol.9 No.3,
August
9,
2018
ABSTRACT: Background: Alpinia
officinarum Hance is valued as an edible
medicinal plant. The rhizome is widely reported to have anticancer activity
whereas little information is available on the aerial part. This study
investigates chemical composition, proapoptotic and anti-osteoporosis
activities of essential oil from aerial parts of A. officinarum (APEO). Methods: In this study, APEO was extracted by hydrodistillation and
analyzed using GC-MS. The inhibitive
activity of 0 - 2.5 μL/mL. APEO was investigated using
MTT assay, while in vivo effect was evaluated in nude
mice. The cell cycle, apoptosis, Δψm
and expression of proteins analyses influenced by 0 - 0.313 μL/mL
APEO were detected by PI, Annexin V/PI, JC-1, and Western blot, respectively. Alkaline
phosphatase activity and mineralized nodules formation of rat osteoblasts with
0 - 0.156 μL/mL APEO were assayed using colorimetric method
and alizarin red staining, respectively. Results: Total 45 constituents were identified accounting for 91.1% of
APEO (sesquiterpene hydrocarbons for 44.4%). APEO significantly inhibited cancer
cells growth in a dose-dependent manner. APEO also inhibited cancer growth in vivo. The percentage of S phase cells
is up to 64.846% with 0.313 μL/mL APEO. The
proportion of total apoptotic cells significantly increased to 79.6% at 0.313 μL/mL concentration. APEO
treated cells accompanied with Bcl-2 and Δψm decrease, and caspase-3
and p53 upregulation. Furthermore, addition of APEO in rat osteoblasts led to a
dose-dependent increase in ALP activity and formation of mineralized bone nodules. Conclusions: Our data suggest that APEO could be developed as an
agent against human lung cancer and osteoporosis, especially cancer-induced
bone loss.