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has been cited by the following article:

• TITLE: Anti-Tubercular Activities and Molecular Characterization of Salivary Extract of Leech (Hirudo medicinalis) against Mycobacterium tuberculosis

  AUTHORS: Praise Olufunmilola Ojo, Hausatu Babayi, Israel Kayode Olayemi, Oladosun Oluwale Peter, Labake Ajoke Fadipe, Elisha Baba, Kasim Izebe

  KEYWORDS: GC-MS, Mycobacterium tuberculosis, Leech Salivary Extract

  JOURNAL NAME: Journal of Tuberculosis Research, Vol.6 No.1, March 6, 2018

  ABSTRACT: Leech salivary extract (LSE) was screened for the anti-tubercular activity against Mycobacterium tuberculosis using microbroth dilution method. The bioactive components in LSE were determined using Shimadzu GC-MS QP2010 SE. The LSE exhibited bactericidal effects on M. tuberculosis at 50% (v/v). The result of the GC-MS analysis of LSE revealed seventeen (17) bioactive components. The major fatty acid components were oleic acid (33.9%) and palmitic acid also known as hexadecanoic acid (22.6%). Other bioactive components detected in appreciable amounts in LSE include, 4-bromobutyric acid (16.86%), 6,17-Octadiene-1-ol acetate (9.78%) and octahydro-1,4,9,9- tetramethyl (5.21%). The anti-tubercular activity of the LSE was attributed to these bioactive components. The results obtained suggest that leech salivary extract could be used in treating tuberculosis.