TITLE:
Fermented Herbal Decoction Selectively Targeting Human Cancer Cell Line and Human Pathogenic Microorganism
AUTHORS:
Nobuo Yamaguchi, Nurmuhammat Amat, Kazuhiro Okamoto, Tsugiya Murayama
KEYWORDS:
Fermented Herbal Decoction, Human Malignant Cell Line, Human Normal Cell Line, Anti-Virus Activity, 3-Methylholanthrene, Experimental Colon Can-cer, In Vitro, In Vivo Anti-Cancer Trial
JOURNAL NAME:
Open Journal of Rheumatology and Autoimmune Diseases,
Vol.8 No.1,
February
2,
2018
ABSTRACT:
Introduction: Prolonged immuno-suppressed status
promised to induce internal growth of malignant cell and infectious agent, yet,
only a small part of affected individuals seek medical attention or berried by
commercially over-flowed fake information.
Several studies have described complementary and alternative medicine as
effective strategies for improving anti-infectious agent including malignant
cell. The purpose of this study was to investigate the effect of a fermented
herbal decoction (FHD) both in vitro and in vivo to malignant cells and
microorganism by regulating leukocyte subset proportioning FHD as dietary
material. Methods: In this approach of alternative study, selective
anti-cancer effect by fermented decoction was tried to show first in vitro system both, cancer cell and
virus strain. The fermented herbal decoction consisting of 80 sorts of herbs
and fruits. The selective toxicity was set up and then for immunological
factors in animal and human. The most important factor is to reduce side effect
for a normal cell. Results: First, FHD was proved as safe by animal test.
FHD regulated also the proportion of granulocyte and lymphocyte ratio both
animal and human. In vitro culture
showed selective toxicity by FHD against human melanoma and leukemia cell line
but reduced toxicity was showed by normal cell line. As for the anti-virus
activity, anti-virus effect was tested on the feeder layer of human fibroblast
cell, after 9 days of culture. Second, FHD inhibits colon cancer growth in
3-methylholanthrene induced cancer in rat. Conclusion: The present
results suggest that our fermented herbal decoction showed selective
anti-cancer activities and anti-virus activities, together with the regulative
effect on the immune system.