TITLE:
Expression of Mammaglobin and Carcinoembryonic Antigen in Peripheral Blood of Patients with Breast Cancer Using Real Time Polymerase Chain Reaction
AUTHORS:
Negin Khosravi, Naghmeh Bahrami, Adnan Khosravi, Atefeh Abedini, Arda Kiani, Somayeh Sharifynia, Pegah Gharaeeyan, Sharareh Seifi, Abdolreza Mohamadnia
KEYWORDS:
Breast Cancer, Biomarker, Mammaglobin mRNA, Carcinoembryonic Antigen mRNA
JOURNAL NAME:
Open Journal of Clinical Diagnostics,
Vol.7 No.4,
December
1,
2017
ABSTRACT: Background
and Objectives: Breast cancer is among the most common causes of
cancer related mortality in women worldwide. Early detection and prompt
diagnosis of tumor is the first step to prevent cancer-related morbidity and
mortality, and a comprehensive understanding of the involved molecular
mechanisms can greatly help in this respect. Breast cancer, like many other
types of cancer, is caused by a combination of genetic and epigenetic changes
such as inactivation of tumor suppressor genes. Materials and Methods: This
study was performed on 40 breast cancer patients and 40 healthy controls.
Quantitative real time reverse transcription polymerase chain reaction (real
time qRT-PCR) was used to assess the expression of carcinoembryonic antigen
(CEA) and mammaglobin mRNA in the peripheral blood of patients and healthy
controls. The two groups were compared using t-test. Results: The two
groups were not significantly different in terms of the mean age. Twenty-nine out of 40 cancer patients were
positive for CEA mRNA and its sensitivity was calculated to be 72.5%.
Twelve out of 40 healthy controls were positive
for CEA mRNA. Twenty-six out of 40 patients were positive for mammaglobin mRNA
indicative of 65% sensitivity while only five out of 40 healthy controls were
positive for mammaglobin mRNA. Conclusion: Both CEA and mammaglobin mRNA
had high sensitivity in cancer patients; thus, they can be used for screening
and early detection of breast cancer patients. Further studies with larger
sample sizes are required to confirm the current findings.