TITLE:
The Transcriptional Activity of LAMP3 Gene Involved in Autophagocytosis in Colorectal Cancer LAMP3 Expression in Colorectal Cancer
AUTHORS:
Martyna Bednarczyk, Małgorzata Muc-Wierzgoń, Nikola Zmarzły, Celina Kruszniewska-Rajs, Beniamin Grabarek, Agata Kaźmierczak, Bartłomiej Skowronek, Klaudia Simka, Dariusz Waniczek, Katarzyna Walkiewicz, Urszula Mazurek
KEYWORDS:
Autophagy, Colorectal Cancer, LAMP3, Microarrays, miRNA
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.5 No.12,
November
21,
2017
ABSTRACT:
Background: Autophagocytosis is a biological process involving the controlled distribution of cell fragments and organelles in order to obtain an additional source of energy. LAMP3 (lysosome-associated membrane protein 3) is a heavily glycosylated integral membrane protein located mainly in the lysosomal membrane. Recent studies have shown that it participates in tumor metastasis and drug resistance. Its main role is contribution to tumor cells proliferation, migration and invasion. The aim of this study was to determine changes in transcriptional activity of LAMP3 at various stages of colorectal cancer. In addition, an attempt was made to select miRNAs potentially regulating LAMP3 expression using bioinformatic databases. Methods: The study was conducted on healthy colon samples and colon cancer samples in four stages. Molecular analysis included the extraction of total RNA, purification of the obtained extracts, expression profile analysis using oligonucleotide microarray technique and in silico determination of miRNAs potentially regulating the LAMP3 expression. Results: The level of LAMP3 expression is higher in colorectal adenocarcinoma cells than in non-cancerous cells and depends on the stage of the disease. Conclusions: LAMP3 may promote cancer progression, metastasis and cause the resistance to treatment.