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Article citations


Crowther, J.R. (2009) Tritation of Reagents. In: Crowther, J.R., Ed., The ELISA Guidebook (Methods in Molecular Biology), 2nd Edition. Humana Press, New York City, 79-109.

has been cited by the following article:

  • TITLE: Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease

    AUTHORS: Alejandra Larsen, Santiago Corva, Javier Panei, Christoph Geisler, Eduardo Mortola

    KEYWORDS: Bovine Leukemia Virus, Indirect Dual ELISA, Recombinant Antigens

    JOURNAL NAME: Open Journal of Animal Sciences, Vol.7 No.3, July 6, 2017

    ABSTRACT: Enzyme-linked immunosorbent assay (ELISA) is often used to test bovine leukemia virus (BLV) infection. However, commercially available kits test in South America detect only antibodies against the gp51 protein. With the aim to improve the sensitivity of the test, we developed here a two-step indirect dual ELISA test that included both proteins p24 and gp51, expressed and produced in E. coli and baculovirus expression system respectively. Two hundred ten BLV sera, stated as double positive or double negative by the combination of commercial agar gel immunodiffusion (AGID) assay and a gp51-ELISA test, were tested with our in house dual rp24/rgp51 ELISA. Firstly, we checked the purified, optimized and standardized proteins as antigen by the checkerboard technique, and set up our in house ELISA test. The concordance correlation coefficient (CCC) and coefficient of variation (CV) intraplate repeatability levels were within the values established by the international standards. The statistical analysis demonstrated the value of sera correctly ranked highest (93.48%), and for 0.3 cutoff, the sensitivity was 95.65% and the specificity 91.30%. In conclusion, the rp24/rgp51 ELISA developed and standardized here demonstrated to have good analytical characteristics to be considered for screening of BLV.