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has been cited by the following article:

• TITLE: An Improved High Performance Liquid Chromatographic Method for Identification and Quantization of Polyamines as Benzoylated Derivatives

  AUTHORS: Rajat Sethi, Sai Raghuveer Chava, Sajid Bashir, Mauro E. Castro

  KEYWORDS: Polyamine, RP-HPLC, Standardization, Benzoylation

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.2 No.4, August 31, 2011

  ABSTRACT: A simple reversed phase HPLC method was developed for the determination of Putrescine, Cadaverine and Spermidine (a class of polyamines) in their benzoylated form from external known standards. In the optimization procedure, a number of parameters were examined: 1) Solvent used in the extraction of standard polyamines (diethyl ether versus chloroform); 2) Solvent used in the elution of the polyamine (methanol versus acetonitrile); 3) Mode of derivatization and extraction step(s) (derivatization and extraction performed together versus derivatization and extraction performed separately); and 4) Other instrumental parameters (such as UV detection wavelength, gradient profiles). The advantages of our method, relative to the standard Morgan method are: a) decreased chromatographic runtime, b) ease of preparation with good resolution, sensitivity, and reproducibility using a standard RP-HPLC method.