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Petsikos-Panayotarou, N., Markellou, E., Kalamarakis, A.E., Kyriakopoulou, D. and Malathrakis, N.E. (2003) In Vitro and in Vivo Activity of Cyprodinil and Pyrimethanil on Botrytis cinerea Isolates Resistant to Other Botryticides and Selection for Resistance to Pyrimethanil in a Greenhouse Population in Greece. European Journal of Plant Pathology, 109, 173-182.
ABSTRACT: The effects of biofungicide formula containing the biocontrol agent Trichoderma harzianum (Jn14) as wettable powder in combination with the fungicides pyrimethanil and a mixture of cyprodinil and flydioxonil on Botrytis cinerea of strawberry in vitro, in vivo and in greenhouse were studied. The pathogen B. cinerea was more affected in vitro by low concentrations of the fungicides tested above 0.4 μg·ml-1 than the bioagent T. harzianum (Jn14). The later was almost insensitive to pyrimethanil. In the same direction, gray mould disease severity on strawberry detached leaves and whole plants was reduced up to 89% by the tested fungicides, compared to the control, while the application of T. harzianum (Jn14) alone reduced disease severity up to 45% on strawberry detached leaves, compared to the control. In the integrated control approach, the combination of T. harzianum (Jn14) with higher concentrations of the tested fungicides (ED50) completely inhibited strawberry gray mould disease severity in pots and the greenhouse.