TITLE:
The Combined Action Strategy of Two Stresses, Salinity and Cu++ on Growth, Metabolites and Protein Pattern of Wheat Plant
AUTHORS:
Hamdia M. Abd El-Samad, D. Mostafa, Kholoud N. Abd El-Hakeem
KEYWORDS:
Osmotic Stress Levels, Cupper, Tolerance, Wheat, Protein Bands
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.3,
February
28,
2017
ABSTRACT: The response of wheat plants to different osmotic
stress levels varied among the different organs root, shoot and spike and the
situation of these organs with application of two Cu++ levels 5 mM
and 25 mM as CuSO4. The sensitivity of root organ was related with
reduction in fresh, dry matter and length. This resulted from reduction of
soluble sugar reflected a reduction in water uptake and K+ content
of the cell sap. In the moderate organ spike, the reduction in fresh, dry
matter and length were concomitant with the accumulation of soluble sugar and a huge
accumulation of soluble protein. In the higher organ shoot, this related with
more water uptake which in turn induced an accumulation of soluble protein and
cofactor K+ content. It can
be recorded that shoot was higher Na+ accumulation than root and
spike. Data
also showed further stimulatory effect on growth parameters by Cu++ applications with either concentration (7.5 mM and 25 mM). Irrigating the soil with either 7.5
or 25 mM CuSO4 induced a huge accumulation in soluble sugar, soluble
protein and nitrate reductase. Cupper treatment with either concentration 7.5
mM or 25 mM induced a marked decrease in Na+ content at all OSL and
has no significant change in the accumulation of K+ in both shoot
and spike whereas induced a huge accumulation in root organ. The synthesis of protein
bands with molecular weight 32.3 KDa at -1.5 MPa NaCl level treated with either 7.5 mM or 25 mM Cu++ concentrations was induced. Also the appearance of protein band with molecular
weight 37KDa induced only at Cu++ treatments with 25 mM concentration
in both control and under different osmotic levels (0.0, -0.3
MPa, -0.9
MPa, -1.5 MPa NaCl).